Evaluation of antioxidant activity of some plant extracts and. Ephedra alata aerial parts were collected in july 2014 from the mountains of jenin region of west bank palestine. Pdf methods for determining the antioxidant activity. There is growing interest in correlating the phytochemical constituents of a plant with its pharmacological activity. Quantification of the antioxidant activity of plant extracts. Pdf on apr 24, 2014, manzoor ahmad shah and others published plant extracts as natural antioxidants in meat and meat products find. In this study, the dpph free radical scavenging activity of the extracts of marsilea quadrifolia is analysed. Oxidation process is one of the most important routes for producing free radicals in food, drugs and even living systems pourmorad et al. We determined the total phenolic and flavonoid content by. The obtained results showed that the extracting solvent significantly influenced the antioxidant property estimations of halimium halimifolium. Thepurpose of this study was to evaluate the antioxidant substances,antioxidant activity, and protection of dna from oxidative damage in humanlymphocytes induced by hydrogen peroxide h2o2. Evaluation of antioxidant capacity of plant extracts by. Difference of solvent polarity to phytochemical content. Aug 19, 2015 then, cluster analysis was useful and allowed the selection of best plant extracts based on the results of different antioxidant capacity methods, giving that, among the plant extracts evaluated, the best results were obtained for oregano, marjoram, lemon balm and rosemary.
Karthika abstract the aim of present study was to estimate the total phenolic and flavonoid contents in vitro antioxidant potential of methanolic leaf and root extracts of the herb. Antioxidant activities and estimation of the phenols and. A detailed study was performed on the antioxidant activity of the methanol extract of whole. Plant extracts, foodstuffs and some beverages are now regarded as important sources of dietary antioxidant, exerting positive effects on human health and in the aging process dorman et al. Inhibition of microsomal peroxidation data represents, mean sem, for n3 the reducing power of different commercial extracts was performed and showed. Apple extracts two varieties showed also strong antioxidant activity even though the total phenolic contents were low gae willow bark, spruce needles, pine bark and cork, and birch phloem, and in some medicinal plants including heather, bogrosemary, willow herb, and meadowsweet. Ten microliters of 10 mgml plant extracts were loaded on a thin layer chromatography plates. High values total phenolic content of both the methanolic and aqueous extracts were observed respectively, in. These natural plant antioxidants can therefore serve as a. Antioxidant and bactericidal activity of wild turmeric extracts rachana s.
Research article open access antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts torilis leptophylla l naima saeed, muhammad r khan and maria shabbir abstract background. The extract from carrot was more heatstable than other extracts. Lin, screening of brazilian plant extracts for antioxidant activity by the. Polyphenols antioxidant analysis for chemical analysis of polyphenols, appropriate dilution of sample powder of plant organ is usually measured and used as aliquot. The ethanol is a recommended solvent for extracting antioxidants from this plant. Evaluation of antioxidant activity of medicinal plant. Some earlier workers have also found good correlation in phenolic content and antioxidant activity of plant extract.
Aframomum melegueta schum zingiberaceae is a perennial herb widely cultivated for its valuable seeds in the tropical region of africa. Indeed, for use in food and nutraceuticals, aqueous plant extracts are nutritionally more relevant and would have obvious advantages in relation to certi. The antioxidant effects were evaluated using in vitro, 2, 2diphenylpicrylhydrazine photometric assay and in vivo serum catalase, superoxide dismutase. Nov 26, 20 herbaceous plants containing antioxidants can protect against dna damage. These extracts presented the highest phenolic content 300. Evaluation of two methods for the extraction of antioxidants.
Comparative analysis of the antioxidant and freeradical. Mar 27, 2007 the efficiencies of two traditional extraction methods used in chinese medicine the decoction method and the maceration method were evaluated for the extraction of antioxidants from medicinal plants. Comparison of in vitro antioxidant properties of extracts from three. Phytochemicals like carotenoids, tocopherols, ascorbates and phenols present in the plants are strong antioxidants and have an important role in the health care system. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. It was found that oak quercus robur, pine pinus maritima, and cinnamon cinnamomum zeylanicum aqueous extracts possessed the highest antioxidant capacities in most of the methods used, and thus could be potential rich sources of natural antioxidants. Screening of in vitro antioxidant activity of methanolic. Natural antioxidants in foods and medicinal plants. Antioxidant activities and phytochemicals of leaf extracts from 10. Antioxidants play an important role to protect damage caused by oxidative stress os. Extraction methods of antioxidants from foods and medicinal plants.
Plant extracts as natural antioxidants in meat and meat. Antioxidant and bactericidal activity of wild turmeric extracts. Superoxide radical scavenging activity data represents, mean sem, for n3 figure 3. Comparative study of antioxidant properties and total. Preliminary analysis of brazilian amazonian plant extracts. Liquid mixed 54 tocopherols are available on the market diluted with vegetable oil or prepared as synergistic mixtures with 55 rosemary extracts, ascorbyl palmitateascorbic acid, or other antioxidants and synergists such as lecithin and. Sep 01, 2014 read plant extracts as natural antioxidants in meat and meat products, meat science on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Research article open access antioxidant activity, total.
Study on antioxidant property in selected medicinal plant extracts. The aim of this study was to screen various solvent extracts of whole plant of torilis leptophylla to. Free radical scavenging and antioxidant impact of indian medicinal plant extracts on h2o2 mediated oxidative stress on human erythrocytes syed ana jawaid, sanjoli jain, manisha bhatnagar, sharmishtha purkayastha, sabari ghosal and anupama sharma avasthi. A group of medicinal plants possessing nutritious and tonic functions were chosen as model plants. The total content of phenolic compounds in the extracts was determined by the folinciocalteau method. Original article antioxidant and free radical scavenging effect of morinda citrifolia fruit extract vennila srinivasahan 1 and brindha durairaj2 1 department of biochemistry, psg college of arts and science, coimbatore, tamil nadu, india, 2department of biochemistry, psg college of arts and science, coimbatore, tamil nadu, india. Antioxidant activity aoa of plant extracts was also measured in widely used for this purpose carotenemethyl linoleate cooxidation system dapkevicius et al. Assessment of antioxidant activity of plant extracts by. Antioxidant activity of some plant extracts of the family. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of hypochaeris radicata l.
Oregano extract was found to be the most effective in stabilizing lard, followed by thyme, dittany, marjoram and lavender extracts, in a decreasing order. Plant secondary compounds have some activities like antioxidant, antimicrobial, and antibiofilm. Pdf on apr 24, 2014, manzoor ahmad shah and others published plant extracts as natural antioxidants in meat and meat products find, read and cite all the research you need on researchgate. Antioxidant properties of the methanol extracts of the leaves.
Polyphenolic extracts obtained from different plant. Assessment of antioxidant activity of plant extracts by different methods p. Plant polyphenols as dietary antioxidants in human health and disease. Antioxidant polyphenolrich extracts from the medicinal. The three extracts stored in the dark at 5 and 25 c after a 15 day period did not show any significant change p. Jan 01, 2014 read phenolic compounds and plant phenolic extracts as natural antioxidants in prevention of lipid oxidation in seafood. Plant material extracts were studied by in vitro methods, such as total phenolic. Several assays have been frequently used to estimate antioxidant capacities in plant extracts including dpph 2,2. To investigate the total phenol content, the total flavonoid content and antioxidant properties of ethanolic and aqueous extracts of the whole plant of euphorbia hirta methodology. Hydroalcoholic extract of kalanchoe pinnata was optimized in ratio of methanol to water 1. Methanol and ethanol have been extensively used to extract antioxidant compounds from various plants and plantbased foods fruits, vegetables etc. Investigation of in vitro and cytotoxic activity of. Antioxidants from plant based origin have gained much interest in recent years as they can reduce the harmful effects of oxidative stress without the undesirable side effects.
Evaluation of the in vitro and in vivo antioxidant. Preliminary analysis of brazilian amazonian plant extracts using benchtop assays in a throughput basis aiming the identification of potential antioxidant natural product plants are a source of compounds that are used for the treatment of human and veterinary diseases. Research article assessment of total phenolic and flavonoid. Most of the antioxidant compounds in a typical diet are derived from plant sources and belong to. Antioxidant properties of the methanol extracts of the leaves and stems of celtis africana adeolu a. Therefore, the present study investigates the content of total phenolics, flavonoids and the antioxidant. Hence, there is a need for development of safer antioxidants particularly from natural sources. Effects of plant extracts on antioxidant status and. The content of total phenolics in the extracts was determined spectrometrically according to the folin. These data indicate that selected plant extracts are potential sources of dietary antioxidants. Pdf study on antioxidant property in selected medicinal plant. Masika3 1department of veterinary physiology, biochemistry and pharmacology, university of ibadan, nigeria.
Recently there has been an upsurge of interest in the therapeutic potentials of medicinal plants as antioxidants in reducing such free radical induced tissue injury. Phytochemical analysis, antioxidant and antilipid peroxidation. Total phenolic, total flavonoid, tannin content, and. In our study, the four methods used to quantify the antioxidant activity of the 12 selected species showed the. Antioxidant activity, total phenolic and total flavonoid. Plant extract activities as antioxidant and antibiofilm. Antioxidant potential was found to be more in extract as compared to control. Pdf free radical scavenging and antioxidant impact of. Decalepis hamiltonil is a plant from asclapiadaceae family. Plant extracts and tbhq significantly decreased lipid oxidation of the goat meats. The solvent was removed under a stream of cold air at room temperature. Each of the plant extracts was added into the refined canola oil 10 g and its purified tags 7 g at concentrations of 250, 500, 1,000 and 2,000. Addition of plant extracts into refined canola oil and its tags. Antioxidant activity and total phenolic compound content.
Preliminary phytochemical screening, quantitative estimation of total flavonoids, total phenols and antioxidant activity of ephedra alata. Figure 1 shows the amount of each extract required for 50% inhibition of dpph activity ic 50. Evaluation of antioxidant activity of medicinal plant 885 figure 2. Ourmethods used acidic methanol and water extractions from six herbaceousplants, including bidens alba ba, lycium chinense lc. The present study was designed to investigate the antioxidant properties and phenolic contents total phenols, flavonoids, flavonols and proanthrocyanidins of methanolic extracts from morus alba locally named as tut and. Antioxidant activity of plant extracts containing phenolic.
Antioxidant activity and total phenolic compound content of certain medicinal plants essam y. Pdf plant extracts as natural antioxidants in meat and meat products. Oct 20, 2014 being natural products, both pure phenolic compounds, as well as plant polyphenolic extracts, have been increasingly preferred over synthetic antioxidants for the reasons of being nontoxic and posing no health concerns. Determination of total antioxidant capacity total antioxidant capacities of the extracts were determined by the phosphomolybdenum method24. Effect of extraction solventtechnique on the antioxidant. Vol6, issue 4, december 2014 january 2015, 850855 e 852 leaves extract contained phytochemical compounds that were showed at table 2. Then, cluster analysis was useful and allowed the selection of best plant extracts based on the results of different antioxidant capacity methods, giving that, among the plant extracts evaluated, the best results were obtained for oregano, marjoram, lemon balm and rosemary. Antioxidant properties of extracts from selected plant materials. The concentration of extracts in lard varied from 0. In this way, we have investigated the antioxidant activity of extracts from gevuina avellana and rosa rubiginosa defatted seeds and hulls 2 4. Plant extracts prepared from plant materials are rich in phenolics and provide a good alternative to synthetic antioxidants.
Journal of agricultural and food chemistry 2014, 62 46, 1114611156. Plant extracts prepared from different plant materials are rich in phenolics and provide a good alternative to synthetic antioxidants. Epidemiological studies and associated metaanalyses strongly suggest that long term consumption of diets rich in plant polyphenols offer protection against development of cancers, cardiovascular diseases, diabetes. Some plant extracts used as antioxidants, such as from grape seeds. Over the years, research on antioxidants, as potential therapeutic agents to prevent free radical generated damage in the human body, has gained popularity. Antioxidants of natural origin, compared to the synthetic antioxidants present in the market, have attracted considerable attention by consumers and by researchers since there is concern of.
The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials berries, fruits, vegetables, herbs, cereals, tree materials, plant sprouts, and seeds was examined by autoxidation of methyl linoleate. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. The preliminary screening of the various extracts was carried out using standard methods and the results. The antioxidant activity of plant extracts can be quantified by different methods of measurement. The antioxidant effects of various plant extracts, as well as individual phenolic compounds, have been evaluated and tested in different seafood model systems. Solvent extraction is most frequently used technique for isolation of plant antioxidant compounds. Rhizophora mangle is a plant from rhizophoraceae family. In vitro assessment of cytotoxicity, antioxidant, and anti. Free radical scavenging and antioxidant impact of indian. Effects of some plant extracts on the oxidative stability. Sham prasad biomedicinal research laboratory, vidya herbs pvt.
Antioxidant activity determination of citronellal and. Plant sourced food antioxidants like vitamin c, vitamin e, carotenes, phenolic acids and phytoestrogens have been recognized as having the potential to reduce disease risk. Lightfoot 2 1 department of food science, college of agriculture, university of albasrah, basrah 61004, iraq 2 department of plant, soil and agricultural systems, plant biotechnology and. Antimicrobial and antioxidant activity of leaf and flower.
Antioxidant and bactericidal activity of wild turmeric. The free radical scavenging action of methanol extracts of plant are in the order as desmodium gangeticum amaranthus caudatus solanum nigrum. Preparation strategies and characterization of plant phenolics samples 2, 8. Dpph free radical scavenging activity of the extracts of the aquatic fern. Total phenolic content was also determined by the folin. Phytochemical screening, antioxidant and antimicrobial. In this work, the antioxidant activity of dried rhizomes extract of the spice curcuma aromatica wild. Evaluation of antioxidant activity of medicinal plant extracts produced for commercial purpose a. Grape seed, green tea, pine bark, rosemary, pomegranate, nettle and cinnamon have exhibited similar or better antioxidant properties compared to some synthetic ones. However, the extract yields and resulting antioxidant activities of the plant materials are strongly dependent on the nature of extracting solvent, due to the presence of different antioxidant compounds. Evaluation of antioxidant activity and total phenol in. Phenolic content is greater in ethanolic extract of both plant parts than its aqueous extract which might be responsible for high antioxidant activity and reducing power of respective extract. The antioxidant activity of the plant extracts and the tbhq ranged from 4. The use of plant extracts to combat infections or illnesses may be explained by the presence of antimicrobial compounds, but there is also another explanation.
The aim of this study was to screen various solvent extracts of whole plant of torilis leptophylla to display potent antioxidant activity in vitro and in vivo, total phenolic and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. Many phenolic compounds and plant phenolic extracts have successfully demonstrated the retarding effect on lipid oxidation in different seafoods. The bark extract of the plant showed scavenging activity of hydroxyl radicals and the extract contained polyphenols. Role of plant polyphenolic extracts as potential antioxidants in fish muscle. If plant extracts contain antioxidant activity, it may clearup free radicals from the body, and boost the immune system to handle the infection or illness effectively. Jayachitra and krilhiga, 2010, antioxidants have been reported. A comparative study on the antioxidant activity of.
The antioxidant activities of methanol extracts of oregano, dittany, thyme, marjoram, spearmint, lavender and basil were tested in lard stored at 75c. The antioxidants present in the plant extracts react with oxidizing agents and their potential to. This research was conducted to obtain the plant with the highest activity of antibiofilm and also antioxidant by analyzing several plant secondary compounds as antioxidant and. In the last decade, there has been much interest in the potential health benefits of dietary plant polyphenols as antioxidant. Plant derived antioxidants such as polyphenols including phenolic acids, phenolic diterpenes, flavonoids, catechins, coumaric and rutin are becoming progressively more important as dietary factors. All plant extracts, except for tarragon, retarded peroxide formation at 80c the most effective being sage and sweet grass extracts fig. Plant polyphenols as dietary antioxidants in human health. Rhododendron, one of the most famous ornamental plants in the world. Further, higher level addition of antioxidants was more effective in. Studies on phenolic composition and antioxidant action of vegetable extracts have been reported recently. Various plants have been investigated to identify the presence of phenolic compouds using hplc methods. Pdf plant extracts as natural antioxidants in meat and. The aim of the work is to ascertain the level of antimicrobial and antioxidant properties of different solvent extracts of senna occidentalis plant.
Screening of medicinal plant extracts for antioxidant activity. Polyphenols antioxidant analysis for chemical analysis of polyphenols, appropriate dilution of sample powder of plant. Antioxidant activity of plant extracts and their effect on. The total phenolic contents tpc and the antioxidant activities of water and ethanol extracts of 14 medicinal plants which had been used in iraqi traditional medicine were investigated.
Plant extracts rich in polyphenols and essen tial phytoconstituents have been shown to display potent antioxidant and free radical. Venugopalan abstract naturally occurring antioxidants have considerable importance in medicine and in food processing. Dpph free radical scavenging activity of the extracts of. Consumption of herbs and spices has been implicated in the prevention of cardiovascular diseases, carcinogenesis, inflammation, atherosclerosis, etc. The study of phenolic compounds antioxidant activity in. A waters 2690 alliance waters, milford, ma, usa separation module equipped with a supelcosil lc18 analytical 5 m, 150 mm. Plant extracts were reconstituted using acetone to a final concentration of 10 mgml. Aqueous extracts of 30 plants were investigated for their antioxidant properties using dpph and abts radical scavenging capacity assay, oxygen radical absorbance capacity orac assay, superoxide dismutase sod assay, and ferric reducing antioxidant potential frap assay. The efficiencies of two traditional extraction methods used in chinese medicine the decoction method and the maceration method were evaluated for the extraction of antioxidants from medicinal plants. Antioxidant polyphenolrich extracts from the medicinal plants antirhea borbonica, doratoxylon apetalum and gouania mauritiana protect 3t3l1 preadipocytes against h 2 o 2, tnf. Studies have been taken up to evaluate the antioxidant potentials in plant extract by various means. The effect of various plant extracts was measured on several important oxidation indicators. Many studies have demonstrated the efficacy of plant derived products as antioxidants against various diseases.
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